| Teaching Staff
|
Öğr.Gör. Çiğdem EKİN |
| Language of Instruction |
Türkçe (Turkish) |
| Type Of Course |
Vocational |
| Prerequisites |
Compulsory |
| Recommended Optional Programme Component |
|
| Course Objectives |
Give the theoretical and practical gains in immunohistochemistry techniques
|
| Course Content |
This course includes; defines the basic techniques of Immunohistochemistry, defines antibodies in immunohistochemistry, defines tumor antigens, basic enzymology, defines and applies the techniques of fixation in immunohistochemistry, explain the steps of immunohistochemical staining technics (applies antigen retrieval step, prepare control preparations, defines and implements the methods of painting) ıdentifies ımmunofluorescence techniques, identifies the problems encountered in immunohistochemistry |
| Learning Outcomes (LO) |
At the end of this course, students will perform the followings:
1. Define the basic techniques of Immunohistochemistry
2. Define antibodies in immunohistochemistry.
3. Define tumor antıgens.
4. Define basic enzymology.
5. Define and applies the techniques of fixation in immunohistochemistry.
6. Explain the steps of immunohistochemical staining techniques.
6.1. Define and applies antigen retrieval step
6.2.Prepare control preparations.
6.3. Define and implements the methods of staining
7. Identify immunofluorescence techniques
8. Identify the problems encountered in immunohistochemistry. |
| Mode of Delivery |
Face to face |
| Course Outline |
| Week |
Topics |
| 1. Week |
Basic immunohistochemistry |
| 2. Week |
Antibodies in immunohistochemistry
|
| 3. Week |
Tumour antigens |
| 4. Week |
Basic enzymology |
| 5. Week |
Fixation in immunohistochemistry |
| 6. Week |
Antigen retrieval in immunohistochemistry |
| 7. Week |
Oncognes and İmportant panels |
| 8. Week |
MİDTERM |
| 9. Week |
Controls in and Block method in immunohistochemistry |
| 10. Week |
Staining methods in immunohistochemistry |
| 11. Week |
Background staining and the problems |
| 12. Week |
Immunofluorescence technique |
| 13. Week |
Problems encountered in immunohistochemistry |
| 14. Week |
The importance of reading material data sheet |
|
| Assessment |
| |
Percentage(%) |
| Mid-term (%) |
40 |
| Quizes (%) |
|
| Homeworks/Term papers (%) |
10 |
| Practice (%) |
|
| Labs (%) |
|
| Projects/Field Work (%) |
|
| Seminars/Workshops (%) |
|
| Final (%) |
50 |
| Other (%) |
|
| Total(%) |
100 |
|
| Course Book (s) and/or References |
Histotechnology. Carson, F.L., Hladik, C., Cytopreparatory Techniques, ASCP, 2009.
Immunohistochemistry: Basics and Methods. Buchwalow, I.B., Bèocker, W., Springer, 2010.
Diagnostic Immunohistochemistry. Dabbs, D.J., Churchill Livingstone, 2002.
İmmunoloji. Male, D.,Brostoff, J., Roth, D.B., Roitt, I., Palme Yayıncılık, 2009. |
| Work Placement(s) |
|
| The Relationship between Program Qualifications (PQ) and Course Learning Outcomes (LO) |
| P Q 1 | P Q 2 | P Q 3 | P Q 4 | P Q 5 | P Q 6 | P Q 7 | P Q 8 | P Q 9 | P Q 10 | P Q 11 | P Q 12 | P Q 13 | P Q 14 | P Q 15 | P Q 16 | P Q 17 | P Q 18 | P Q 19 | P Q 20 | P Q 21 | P Q 22 | P Q 23 | P Q 24 | P Q 25 | P Q 26 | P Q 27 | P Q 28 | P Q 29 | P Q 30 | P Q 31 | P Q 32 | P Q 33 | LO1 | 2 | 1 | 5 | 0 | 5 | 4 | 0 | 2 | 4 | 3 | 1 | 2 | 1 | 0 | 2 | 4 | 5 | 3 | 1 | 1 | 0 | 0 | 0 | 2 | 0 | 1 | 1 | 0 | 2 | 2 | 2 | 2 | 0 | LO2 | 3 | 2 | 5 | 0 | 5 | 3 | 0 | 3 | 3 | 4 | 2 | 2 | 1 | 0 | 2 | 1 | 5 | 3 | 2 | 1 | 0 | 0 | 0 | 2 | 0 | 1 | 1 | 0 | 2 | 2 | 2 | 2 | 0 | LO3 | 2 | 3 | 5 | 0 | 5 | 3 | 0 | 4 | 4 | 3 | 1 | 2 | 1 | 0 | 2 | 2 | 5 | 3 | 1 | 1 | 0 | 0 | 0 | 2 | 0 | 1 | 1 | 0 | 2 | 2 | 2 | 2 | 0 | LO4 | 2 | 2 | 0 | 0 | 5 | 2 | 0 | 2 | 3 | 4 | 2 | 2 | 1 | 0 | 2 | 1 | 5 | 3 | 2 | 1 | 0 | 0 | 0 | 2 | 0 | 1 | 1 | 0 | 2 | 2 | 2 | 2 | 0 | LO5 | 3 | 1 | 4 | 0 | 5 | 3 | 0 | 3 | 4 | 3 | 1 | 2 | 1 | 0 | 2 | 4 | 5 | 3 | 1 | 1 | 0 | 0 | 0 | 2 | 0 | 1 | 1 | 0 | 2 | 2 | 2 | 2 | 0 | LO6 | 2 | 2 | 4 | 0 | 5 | 3 | 0 | 2 | 3 | 5 | 2 | 2 | 1 | 0 | 2 | 5 | 5 | 3 | 2 | 1 | 0 | 0 | 0 | 2 | 0 | 1 | 1 | 0 | 2 | 2 | 2 | 2 | 0 | LO7 | 2 | 3 | 4 | 0 | 5 | 2 | 0 | 3 | 4 | 3 | 1 | 2 | 1 | 0 | 2 | 2 | 5 | 3 | 1 | 1 | 0 | 0 | 0 | 2 | 0 | 1 | 1 | 0 | 2 | 2 | 2 | 2 | 0 | LO8 | 3 | 2 | 5 | 0 | 5 | 4 | 0 | 4 | 3 | 4 | 2 | 2 | 1 | 0 | 2 | 1 | 5 | 3 | 2 | 1 | 0 | 0 | 0 | 2 | 0 | 1 | 1 | 0 | 2 | 2 | 2 | 2 | 0 |
* Contribution Level : 0 N/R 1 Very low 2 Low 3 Medium 4 High 5 Very High |