Course Title Course Code Program Level
Immunohistochemistry Techniques PLT203 Patoloji Laboratuvar Teknikleri Associate Degree

Course Term
(Course Semester)
Teaching and Learning Methods
Theory Practice Lab Projects/Field Work Seminars/Workshops Other Total Credits ECTS Credits
28 62 20 10 120 2 4

Teaching Staff Yrd.Doç.Dr.Yasemin SEZGİN
Language of Instruction Türkçe (Turkish)
Type Of Course Vocational
Prerequisites Compulsory
Recommended Optional Programme Component
Course Objectives Give the theoretical and practical gains in immunohistochemistry techniques
Course Content This course includes; defines the basic techniques of Immunohistochemistry, defines antibodies in immunohistochemistry, defines tumor antigens, basic enzymology, defines and applies the techniques of fixation in immunohistochemistry, explain the steps of immunohistochemical staining technics (applies antigen retrieval step, prepare control preparations, defines and implements the methods of painting) ıdentifies ımmunofluorescence techniques, identifies the problems encountered in immunohistochemistry
Learning Outcomes (LO) At the end of this course, students will perform the followings: 1. Define the basic techniques of Immunohistochemistry 2. Define antibodies in immunohistochemistry. 3. Define tumor antıgens. 4. Define basic enzymology. 5. Define and applies the techniques of fixation in immunohistochemistry. 6. Explain the steps of immunohistochemical staining techniques. 6.1. Define and applies antigen retrieval step 6.2.Prepare control preparations. 6.3. Define and implements the methods of staining 7. Identify immunofluorescence techniques 8. Identify the problems encountered in immunohistochemistry.
Mode of Delivery Face to face
Course Outline
Week Topics
1. Week Basic immunohistochemistry
2. Week Antibodies in immunohistochemistry
3. Week Tumour antigens
4. Week Basic enzymology
5. Week Fixation in immunohistochemistry
6. Week Antigen retrieval in immunohistochemistry
7. Week Oncognes and İmportant panels
9. Week Controls in and Block method in immunohistochemistry
10. Week Staining methods in immunohistochemistry
11. Week Background staining and the problems
12. Week Immunofluorescence technique
13. Week Problems encountered in immunohistochemistry
14. Week The importance of reading material data sheet
Mid-term (%) 40
Quizes (%)
Homeworks/Term papers (%) 10
Practice (%)
Labs (%)
Projects/Field Work (%)
Seminars/Workshops (%)
Final (%) 50
Other (%)
Total(%) 100
Course Book (s) and/or References Histotechnology. Carson, F.L., Hladik, C., Cytopreparatory Techniques, ASCP, 2009. Immunohistochemistry: Basics and Methods. Buchwalow, I.B., Bèocker, W., Springer, 2010. Diagnostic Immunohistochemistry. Dabbs, D.J., Churchill Livingstone, 2002. İmmunoloji. Male, D.,Brostoff, J., Roth, D.B., Roitt, I., Palme Yayıncılık, 2009.
Work Placement(s)
The Relationship between Program Qualifications (PQ) and Course Learning Outcomes (LO)