Teaching Staff

Öğr.Gör. Çiğdem EKİN 
Language of Instruction 
Türkçe (Turkish) 
Type Of Course 
Vocational 
Prerequisites 
Compulsory 
Recommended Optional Programme Component 

Course Objectives 
Give the theoretical and practical gains in immunohistochemistry techniques

Course Content 
This course includes; defines the basic techniques of Immunohistochemistry, defines antibodies in immunohistochemistry, defines tumor antigens, basic enzymology, defines and applies the techniques of fixation in immunohistochemistry, explain the steps of immunohistochemical staining technics (applies antigen retrieval step, prepare control preparations, defines and implements the methods of painting) ıdentifies ımmunofluorescence techniques, identifies the problems encountered in immunohistochemistry 
Learning Outcomes (LO) 
At the end of this course, students will perform the followings:
1. Define the basic techniques of Immunohistochemistry
2. Define antibodies in immunohistochemistry.
3. Define tumor antıgens.
4. Define basic enzymology.
5. Define and applies the techniques of fixation in immunohistochemistry.
6. Explain the steps of immunohistochemical staining techniques.
6.1. Define and applies antigen retrieval step
6.2.Prepare control preparations.
6.3. Define and implements the methods of staining
7. Identify immunofluorescence techniques
8. Identify the problems encountered in immunohistochemistry. 
Mode of Delivery 
Face to face 
Course Outline 
Week 
Topics 
1. Week 
Basic immunohistochemistry 
2. Week 
Antibodies in immunohistochemistry

3. Week 
Tumour antigens 
4. Week 
Basic enzymology 
5. Week 
Fixation in immunohistochemistry 
6. Week 
Antigen retrieval in immunohistochemistry 
7. Week 
Oncognes and İmportant panels 
8. Week 
MİDTERM 
9. Week 
Controls in and Block method in immunohistochemistry 
10. Week 
Staining methods in immunohistochemistry 
11. Week 
Background staining and the problems 
12. Week 
Immunofluorescence technique 
13. Week 
Problems encountered in immunohistochemistry 
14. Week 
The importance of reading material data sheet 

Assessment 

Percentage(%) 
Midterm (%) 
40 
Quizes (%) 

Homeworks/Term papers (%) 
10 
Practice (%) 

Labs (%) 

Projects/Field Work (%) 

Seminars/Workshops (%) 

Final (%) 
50 
Other (%) 

Total(%) 
100 

Course Book (s) and/or References 
Histotechnology. Carson, F.L., Hladik, C., Cytopreparatory Techniques, ASCP, 2009.
Immunohistochemistry: Basics and Methods. Buchwalow, I.B., Bèocker, W., Springer, 2010.
Diagnostic Immunohistochemistry. Dabbs, D.J., Churchill Livingstone, 2002.
İmmunoloji. Male, D.,Brostoff, J., Roth, D.B., Roitt, I., Palme Yayıncılık, 2009. 
Work Placement(s) 

The Relationship between Program Qualifications (PQ) and Course Learning Outcomes (LO) 
 P Q 1  P Q 2  P Q 3  P Q 4  P Q 5  P Q 6  P Q 7  P Q 8  P Q 9  P Q 10  P Q 11  P Q 12  P Q 13  P Q 14  P Q 15  P Q 16  P Q 17  P Q 18  P Q 19  P Q 20  P Q 21  P Q 22  P Q 23  P Q 24  P Q 25  P Q 26  P Q 27  P Q 28  P Q 29  P Q 30  P Q 31  P Q 32  P Q 33  LO1  2  1  5  0  5  4  0  2  4  3  1  2  1  0  2  4  5  3  1  1  0  0  0  2  0  1  1  0  2  2  2  2  0  LO2  3  2  5  0  5  3  0  3  3  4  2  2  1  0  2  1  5  3  2  1  0  0  0  2  0  1  1  0  2  2  2  2  0  LO3  2  3  5  0  5  3  0  4  4  3  1  2  1  0  2  2  5  3  1  1  0  0  0  2  0  1  1  0  2  2  2  2  0  LO4  2  2  0  0  5  2  0  2  3  4  2  2  1  0  2  1  5  3  2  1  0  0  0  2  0  1  1  0  2  2  2  2  0  LO5  3  1  4  0  5  3  0  3  4  3  1  2  1  0  2  4  5  3  1  1  0  0  0  2  0  1  1  0  2  2  2  2  0  LO6  2  2  4  0  5  3  0  2  3  5  2  2  1  0  2  5  5  3  2  1  0  0  0  2  0  1  1  0  2  2  2  2  0  LO7  2  3  4  0  5  2  0  3  4  3  1  2  1  0  2  2  5  3  1  1  0  0  0  2  0  1  1  0  2  2  2  2  0  LO8  3  2  5  0  5  4  0  4  3  4  2  2  1  0  2  1  5  3  2  1  0  0  0  2  0  1  1  0  2  2  2  2  0 
* Contribution Level : 0 N/R 1 Very low 2 Low 3 Medium 4 High 5 Very High 